Sds Parts
Sds Parts
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Protein analysis questions?
First part:
1) Used SDS page - size of protein: 4kilodalton
2) Used SDS page again - this time using reagents to break disulphide bonds - still 4kilodalton
--> What does this mean? why are the results the same?
--> how many peptide strands should i expect given 36 amino acids from the first SDS- page, which assumed 110 daltons/amino acid (4000/110 ~ 36)
Second part:
protein was analyzed: amino acid sequence as follows:
A^2 C^2 D^2 E^4 F G^3 H L M N^2 P^2 Q^2 R^3 S^4 T^3 W, dansylated-R, and dansylated-K
does this make sense given results from the first part??
Thanks- stumped here!
1) when u run an SDS PAGE, it will separate the proteins (poypeptides of the protein) based on their molecular weight. the molecular weight of ur protein is 4000 daltons or 4kDa
2)braking of disulphide bonds will not change the SDS PAGE picture because it is a picture of individual polypeptides and not its tertiary state which the disulphide bonds give.
3)the amino acid sequence also yielded 36 amino acids which means that it is about 4kDa protein. each letter represents an amino acid.